Anaplasmosis destroys red blood cells in the animal, causing anemia. Affected animals appear weak, pale, and jaundiced, with a high fever. Less specific signs include poor appetite and a sudden, severe drop in milk production. The age of the animal can affect the severity of symptoms. This amplification kit detects Anaplasmosis via Real TimePCR. This allows absolute quantification or a qualitativeassay.Real Time PCR is based on fluorogenic dyes. Ct valuesbetween 12 - 36 should be taken as positive. Values between 36 - 40 Ct should be taken as marginal positive. Ct values above 40 must be considered as negative