Pox diseases are viral diseases that affect many animals, including people and birds. Some poxviruses also cause zoonoses. Typically, lesions of the skin and mucosae are widespread and progress from macules to papules, vesicles, and pustules before encrusting and healing. Most lesions contain multiple intracytoplasmic inclusions, which represent sites of virus replication in infected cells. In some poxvirus infections, vesiculation is not clinically evident, but microvesicles can be seen on histologic examination and, in some, proliferative lesions are characteristic.
This amplification kit detects Pox via Real TimePCR. This allows absolute quantification or a qualitativeassay. Real Time PCR is based on fluorogenic dyes. Ct valuesbetween 12 - 36 should be taken as positive. Values between 36 - 40 Ct should be taken as marginal positive. Ct values above 40 must be considered as negative