Benign theileriosis is characterised by fever, anaemia, tachypnoea, depression, lethargy, jaundice and often eventual death. Exercise intolerance may be observed. Pregnant cows may abort and still-births occur. Death rates are often highest in heavily pregnant cows. This amplification kit detects Theileria via Real TimePCR. This allows absolute quantification or a qualitative assay.
Real Time PCR is based on fluorogenic dyes. Ct values between 12 - 36 should be taken as positive. Values between 36 - 40 Ct should be taken as marginal positive. Ct values above 40 must be considered as negative