Animal trypanosomiasis occurs throughout the tropical regions of Africa and in large areas of Asia and South America. It affects cattle, sheep, goats, pigs, horses, camels, and man. Also, wild animals can be infected with the parasites; however, they seldom suffer from disease. They serve as the reservoir of infection for domestic animals. This amplification kit detects trypanosomiasis via Real TimePCR. This allows absolute quantification or a qualitativeassay.
Real Time PCR is based on fluorogenic dyes. Ct values between 12 - 36 should be taken as positive. Values between 36 - 40 Ct should be taken as marginal positive. Ct values above 40 must be considered as negative