GMOs screening kit for cotton and Textiles is based on IWA 32:2019 (E) Method. The Screening is based on Real time Polymerase Chain Reaction (RT- PCR) method, This Screening kit is covered four major commercial cotton species. Gossypium hirsutum, G.barbadense, G. arboreum G. herbaceum.
GMOs detection kits provide a simple, reliable, and rapid procedure for detecting the presence of a specific target. The assay is based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence in the target organisms or plants.
Genetically modified (GM) food and feed products have become a reality. Since 1994, the date of their initial commercialization, about 150 genetically modified plants have received approval for use as food or feed (1). In most countries, such use is highly regulated with implementation of compliance measures. For the enforcement of this legislation is essential an efficient detection of genetically modified organisms (GMOs) in food and feed products (2). Considering their large diversity (different GM elements in various species), the application of a generic screening for the presence of GM material is most often the first step in GMO analysis (3). Such qualitative screening methods provide a presence/absence response and help reducing the number of subsequent identification analyses. Although promoter 35S obtained from cauliflower mosaic virus (CaMV) and the terminator NOS from Agrobacterium tumefaciens are the most frequent elements present in transgenic material in food, these regulatory elements do not cover such important transgenic events as MON89788 soy, H7-1 sugar beet or GT73 rape. Therefore, in order to ensure a widest screening, it is important to also detect a promotor from Figwort Mosaic Virus (P-FMV) and real-time PCR methods have proved to be a reliable strategy to perform this screening.
