REAL TIME DETECTION KIT Listeria monocytogenes uses real-time PCR technology for the detection of L. monocytogenes in a simple, efficient, reliable, and rapid procedure. This method is based on 5’ nuclease real time PCR reactions to amplify a unique and desirable genomic sequence in the target microorganism. This carefully designed primers and probe ensure highest sensitivity, accuracy and specificity.
Product InsertListeria monocytogenes is a ubiquitous foodborne pathogen that causes listeriosis and is frequently linked to consumption of ready-to-eat (RTE) foods, such as fresh produce, soft cheese, and especially RTE meat products. The disease primarily affects pregnant women, new-borns, older adults, and people with weakened immune systems. It’s rare for people in other groups to get sick with Listeria infection. The ability of Listeria spp. to grow at low temperatures (3ºC) increases the risk of infection. There are several ancient methods to determine and detect the Listeria infection, but they are time taking. In vitro DNA amplification by polymerase chain reaction (PCR), in particular, using real time PCR and specific fluorescent probes, is a powerful and reliable tool for detection of Listeria monocytogenes, providing results much faster and allowing more efficient control procedures.